Combined effects of BARLEYmax™ and cocoa polyphenols on colonic microbiota and bacterial metabolites in vitro
The study reports increases in the relative abundances of phyla Bacteriodetes and Actinobacteria, and the corresponding genera Prevotella and Bifidobacterium with BARLEYmax®. There was also a decrease in pH, an increase in short chain fatty acids (SCFA) and a decrease in alpha-diversity consistent with previous studies. CPP (cocoa polyphenols) significantly increased the alpha-diversity.
BARLEYmax® also caused an increase in the relative abundance in genus Lactobacillus.
On the other hand, BARLEYmax® and CPP supressed ammonia-nitrogen production. Both substrates also decreased the abundance of genus Clostridum, known as an ammonia producer. In this study the concentration of ammonia-nitrogen was positively correlated with the abundance of Clostrium.spp. Ammonia is regarded as toxic and the report cites a study linking ammonia production and tumorigenesis in the colon as opposed to the SCFA which are generally regarded as inhibitory to such tumorigenesis.